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| Natural lupeol level variation among castor accessions and the upregulation of lupeol synthesis in response to light | |
| Li, Donghai1,2; Zaman, Wajid3; Lu, Jianjun2; Niu, Qingqing; Zhang, Xuanhao; Ayaz, Asma; Saqib, Saddam4; Yang, Baiming5; Zhang, Jixing6; Zhao, Huayan; Lu, Shiyou1 | |
| 2023 | |
| 发表期刊 | INDUSTRIAL CROPS AND PRODUCTS
 |
| ISSN | 0926-6690 |
| 卷号 | 192 |
| 摘要 | Lupeol, a plant-derived lupane-type pentacyclic triterpenoid, has many pharmacological activities. Although lupeol is found widely in many fruits, vegetables, and medicinal plants, its low content and difficult extraction limit its current applications. The castor plant (Ricinus communis L.) is a potential candidate plant for producing lupeol because it accumulates at high levels as a dominant wax component in the epidermal surface. The lupeol content varies significantly among castor individuals/cultivars. To identify castor lines for further breeding and uncover the molecular mechanism of lupeol biosynthesis and regulation, several castor varieties with various lupeol levels were identified, including 26 lines with higher lupeol contents and 12 glossy lines with very low lupeol contents. A deletion of the coding sequence of castor lupeol synthase RcLUS-1 was identified in glossy1, which possibly affects the binding ability of the substrate. The role of RcLUS-1 in lupeol production was determined using a virus-induced gene silencing (VIGS) assay. The promoter region of RcLUS-1 was analyzed further to uncover the factors affecting lupeol synthesis. Several potential cis-acting elements responding to light present were enriched in this region. Further study showed that light intensity affects RcLUS-1 expression and lupeol production. Overall, this study evaluated several potential castor resources for future breeding and expanded the knowledge of the lupeol regulation mechanism. |
| 关键词 | Epidermal wax Castor varieties Lupeol synthesis RcLUS-1 Gene regulation |
| 学科领域 | Agricultural Engineering ; Agronomy |
| DOI | 10.1016/j.indcrop.2022.116090 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | OXIDOSQUALENE CYCLASE ; MESSENGER-RNAS ; TRITERPENE ; REQUIREMENT ; EXPRESSION ; SYNTHASE ; DUCKWEED ; ELEMENT ; CLONING |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000934011300001 |
| 出版者 | ELSEVIER |
| 文献子类 | Article |
| 出版地 | AMSTERDAM |
| EISSN | 1872-633X |
| 资助机构 | Natural Science Foundation of China [31760399] |
| 作者邮箱 | lidonghai17@mails.ucas.edu.cn ; shangla123@gmail.com ; jjlu@mju.edu.cn ; 202021107011070@stu.hubu.edu.cn ; 202121107012167@stu.hubu.edu.cn ; asmaayaz@bs.qau.edu.pk ; saddamsaqib.qau@gmail.com ; yangbm660@nenu.edu.cn ; zhangjixing@imun.edu.cn ; huayanzhao@hubu.edu.cn ; shiyoulu@hubu.edu.cn |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/29147 |
| 专题 | 系统与进化植物学国家重点实验室 |
| 作者单位 | 1.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China 2.Hubei Hongshan Lab, Wuhan 430070, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan 430074, Peoples R China 4.Yeungnam Univ, Dept Life Sci, Gyongsan 38541, Gyeongbuk, South Korea 5.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 6.Jilin Agr Sci & Technol Univ, Jilin 132109, Jilin, Peoples R China 7.Inner Mongolia Univ Nationalities, Coll Life Sci & Food Engn, Tongliao 028000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Donghai,Zaman, Wajid,Lu, Jianjun,et al. Natural lupeol level variation among castor accessions and the upregulation of lupeol synthesis in response to light[J]. INDUSTRIAL CROPS AND PRODUCTS,2023,192. |
| APA | Li, Donghai.,Zaman, Wajid.,Lu, Jianjun.,Niu, Qingqing.,Zhang, Xuanhao.,...&Lu, Shiyou.(2023).Natural lupeol level variation among castor accessions and the upregulation of lupeol synthesis in response to light.INDUSTRIAL CROPS AND PRODUCTS,192. |
| MLA | Li, Donghai,et al."Natural lupeol level variation among castor accessions and the upregulation of lupeol synthesis in response to light".INDUSTRIAL CROPS AND PRODUCTS 192(2023). |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 167711973269100.pdf（7787KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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