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| Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice | |
| Guo, Xiaoyu1; Zhang, Dajian1; Wang, Zhongliang1; Xu, Shujuan1; Batistic, Oliver2; Steinhorst, Leonie2; Li, Hao3; Weng, Yuxiang3; Ren, Dongtao4; Kudla, Jorg2; Xu, Yunyuan5; Chong, Kang1,5 | |
| 2023 | |
| 发表期刊 | EMBO JOURNAL
 |
| ISSN | 0261-4189 |
| 卷号 | 42期号:1 |
| 摘要 | Unusually low temperatures caused by global climate change adversely affect rice production. Sensing cold to trigger signal network is a key base for improvement of chilling tolerance trait. Here, we report that Oryza sativa Calreticulin 3 (OsCRT3) localized at the endoplasmic reticulum (ER) exhibits conformational changes under cold stress, thereby enhancing its interaction with CBL-interacting protein kinase 7 (OsCIPK7) to sense cold. Phenotypic analyses of OsCRT3 knock-out mutants and transgenic overexpression lines demonstrate that OsCRT3 is a positive regulator in chilling tolerance. OsCRT3 localizes at the ER and mediates increases in cytosolic calcium levels under cold stress. Notably, cold stress triggers secondary structural changes of OsCRT3 and enhances its binding affinity with OsCIPK7, which finally boosts its kinase activity. Moreover, Calcineurin B-like protein 7 (OsCBL7) and OsCBL8 interact with OsCIPK7 specifically on the plasma membrane. Taken together, our results thus identify a cold-sensing mechanism that simultaneously conveys cold-induced protein conformational change, enhances kinase activity, and Ca2+ signal generation to facilitate chilling tolerance in rice. |
| 关键词 | Calcium signal Calreticulin Cold stress OsCIPK7 OsCRT3 |
| 学科领域 | Biochemistry & Molecular Biology ; Cell Biology |
| DOI | 10.15252/embj.2021110518 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | DNA INSERTIONAL MUTAGENESIS ; PROTEIN-KINASE SOS2 ; ENDOPLASMIC-RETICULUM ; STRESS RESPONSES ; CALCIUM SENSORS ; PLANT-CELLS ; CALCINEURIN ; DOMAIN ; SALT ; CA2+ |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000879368400001 |
| 出版者 | WILEY |
| 文献子类 | Article |
| 出版地 | HOBOKEN |
| EISSN | 1460-2075 |
| 资助机构 | Basic Science Center Project of National Natural Science Foundation of China [31788103] ; National Key Research and Development Program [2020YFE0202300-06] ; Special Research Assistant Project of Chinese Academy of Sciences [2021000017] ; China Postdoctoral Science Foundation [2020M670519] ; Deutsche Forschungsgemeinschaft (DFG) [INST 211/565-1 FUGG, INST211/853-1 FUGG] |
| 作者邮箱 | chongk@ibcas.ac.cn |
| 作品OA属性 | Green Published, hybrid |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/29148 |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Westfalische Wilhelms Univ, Inst Biol & Biotechnol Pflanzen, Munster, Germany 4.Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing, Peoples R China 5.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing, Peoples R China 6.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Xiaoyu,Zhang, Dajian,Wang, Zhongliang,et al. Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice[J]. EMBO JOURNAL,2023,42(1). |
| APA | Guo, Xiaoyu.,Zhang, Dajian.,Wang, Zhongliang.,Xu, Shujuan.,Batistic, Oliver.,...&Chong, Kang.(2023).Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice.EMBO JOURNAL,42(1). |
| MLA | Guo, Xiaoyu,et al."Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice".EMBO JOURNAL 42.1(2023). |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 166787100567624.pdf（5533KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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