ZYGOTE-ARREST 3 that encodes the tRNA ligase is essential for zygote division in Arabidopsis

doi:10.1007/s11676-016-0364-5

	ZYGOTE-ARREST 3 that encodes the tRNA ligase is essential for zygote division in Arabidopsis
	Yang, Ke-Jin ; Guo, Lei ; Hou, Xiu-Li ; Gong, Hua-Qin ; Liu, Chun-Ming
	2017
发表期刊	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
ISSN	1672-9072
卷号	59 期号:9 页码:680-692
摘要	In sexual organisms, division of the zygote initiates a new life cycle. Although several genes involved in zygote division are known in plants, how the zygote is activated to start embryogenesis has remained elusive. Here, we showed that a mutation in ZYGOTE-ARREST 3 (ZYG3) in Arabidopsis led to a tight zygote-lethal phenotype. Map-based cloning revealed that ZYG3 encodes the transfer RNA (tRNA) ligase AtRNL, which is a single-copy gene in the Arabidopsis genome. Expression analyses showed that AtRNL is expressed throughout zygotic embryogenesis, and in meristematic tissues. Using pAtRNL::cAtRNL-sYFP-complemented zyg3/zyg3 plants, we showed that AtRNL is localized exclusively in the cytoplasm, suggesting that tRNA splicing occurs primarily in the cytoplasm. Analyses using partially rescued embryos showed that mutation in AtRNL compromised splicing of intron-containing tRNA. Mutations of two tRNA endonuclease genes, SEN1 and SEN2, also led to a zygote-lethal phenotype. These results together suggest that tRNA splicing is critical for initiating zygote division in Arabidopsis.
学科领域	Forestry
DOI	10.1007/s11676-016-0364-5
收录类别	SCI
语种	英语
WOS关键词	UNFOLDED PROTEIN RESPONSE ; SPLICING ENDONUCLEASE ; MESSENGER-RNA ; SACCHAROMYCES-CEREVISIAE ; FUNCTIONAL DOMAINS ; EMBRYOGENESIS ; THALIANA ; KINASE ; REPAIR ; IDENTIFICATION
WOS记录号	WOS:000404234900016
出版者	WILEY
Special Issue	SI
文献子类	Article
出版地	HOBOKEN
EISSN	1744-7909
资助机构	National Basic Research Program of China from MOST [2014CB943401]
作者邮箱	cmliu@ibcas.ac.cn
作品OA属性	Green Submitted
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/22215
专题	中科院植物分子生理学重点实验室
作者单位	1.[Yang, Ke-Jin 2.Guo, Lei 3.Hou, Xiu-Li 4.Gong, Hua-Qin 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Nanyang Normal Univ, Sch Life Sci & Technol, Nanyang 473061, Peoples R China 8.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Yang, Ke-Jin,Guo, Lei,Hou, Xiu-Li,et al. ZYGOTE-ARREST 3 that encodes the tRNA ligase is essential for zygote division in Arabidopsis[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2017,59(9):680-692.
APA	Yang, Ke-Jin,Guo, Lei,Hou, Xiu-Li,Gong, Hua-Qin,&Liu, Chun-Ming.(2017).ZYGOTE-ARREST 3 that encodes the tRNA ligase is essential for zygote division in Arabidopsis.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,59(9),680-692.
MLA	Yang, Ke-Jin,et al."ZYGOTE-ARREST 3 that encodes the tRNA ligase is essential for zygote division in Arabidopsis".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 59.9(2017):680-692.

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