Probing molecular events associated with early development of thylakoid membranes by comparative proteomics and low temperature fluorescence

doi:10.1016/j.jprot.2016.04.040

	Probing molecular events associated with early development of thylakoid membranes by comparative proteomics and low temperature fluorescence
	Wang, Yangyang 1; Ji, Kuixian ; Shen, Shihua; Chen, Hui
	2016
发表期刊	JOURNAL OF PROTEOMICS
ISSN	1874-3919
卷号	143 页码:401-415
摘要	A comparison of protein profiles between prolamellar bodies from dark-grown etioplasts and thylakoid membranes from de-etioplasts illuminated respectively for 1, 5 and 9 h revealed 155 differentially expressed CBB-stained spots. Clear results showed that the nonphototransformable pchlide(627-632) was the dominant pigment form in the PLBs of rice etioplasts during plant development in dark and transformed slowly to chlorophyllide in rice etioplasts when exposed to light. The light-induced accumulation of ACC oxidase, which catalyzes the final step of ethylene synthesis using ACC as substrate, would facilitate chlorophyll synthesis by inducing PORa/b expression via ethylene signaling. It could be also suggested that cyclic electron transport might play an important role in generation of ATP for carbon fixation and photoprotection of photosystems from excessive light in prothylakoid. Furthermore, the overproduction of ClpC1, which targets proteins to the ClpPR core complex for degradation, was observed only in Stage 1, during which period PLBs disrupted and converted into prothylakoids, suggesting that ClpC1 was of particular importance for disassembly of PLBs of etioplasts when exposed to light. This study revealed the possible biochemical and physiological processes lead to the formation of functional thylakoid membranes. Biological significance: In this study, we monitored the light-induced transformation of prolamellar bodies into thylakoid membranes, which is correlated to the biogenesis of photosynthetic apparatus involving a complex cascade of biochemical and structural events. Three stages of thylakoid development classified according to the thylakoid development status (Adam et al., 2011) were studied for biogenesis of photosynthetic apparatus: Stage 1, prothylakoids emerge from the disrupted PLB5; Stage 2, prothylakoids converted into primary thylalcoids which were dispersed in the stroma; Stage 3, the continuous grana and stroma thylakoids are formed. The development stage-dependent changes in the proteomic profile of the thylakoids were analyzed by two-dimensional electrophoresis (2-DE). This information was complemented with the steady-state 77 K chlorophyll fluorescence of thylakoids at the corresponding development stage. Together, these analyses allowed us to further understand the molecular processes connected to the formation of functional thylakoid membranes. (C) 2016 Elsevier B.V. All rights reserved.
关键词	Thylakoid Development Proteomics Chlorophyll
学科领域	Biochemical Research Methods
DOI	10.1016/j.jprot.2016.04.040
收录类别	SCI
语种	英语
WOS关键词	CYCLIC ELECTRON-TRANSPORT ; SYNECHOCOCCUS SP PCC7942 ; CHLOROPHYLL-A/B-PROTEIN ; PSBA MESSENGER-RNA ; 3 DNAK PROTEINS ; PHOTOSYSTEM-II ; PROTOCHLOROPHYLLIDE OXIDOREDUCTASE ; RUBISCO ACTIVASE ; ENERGY-TRANSFER ; LARGE SUBUNIT
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000381231400036
出版者	ELSEVIER
Special Issue	SI
文献子类	Article
出版地	AMSTERDAM
EISSN	1876-7737
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30971716] ; National Program on Key Basic Research Project (973 program)National Basic Research Program of China [2009CB118500]
作者邮箱	chenh@ibcas.ac.cn
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/24987
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Res & Dev Resource Plant, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yangyang,Ji, Kuixian,Shen, Shihua,et al. Probing molecular events associated with early development of thylakoid membranes by comparative proteomics and low temperature fluorescence[J]. JOURNAL OF PROTEOMICS,2016,143:401-415.
APA	Wang, Yangyang,Ji, Kuixian,Shen, Shihua,&Chen, Hui.(2016).Probing molecular events associated with early development of thylakoid membranes by comparative proteomics and low temperature fluorescence.JOURNAL OF PROTEOMICS,143,401-415.
MLA	Wang, Yangyang,et al."Probing molecular events associated with early development of thylakoid membranes by comparative proteomics and low temperature fluorescence".JOURNAL OF PROTEOMICS 143(2016):401-415.

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