Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation

doi:10.1038/srep19245

	Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation
	Wu, Jie ; Wang, Xiao-Chen ; Liu, Yang ; Du, Hui ; Shu, Qing-Yan ; Su, Shang ; Wang, Li-Jin ; Li, Shan-Shan ; Wang, Liang-Sheng
	2016
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	6
摘要	Flavones are important secondary metabolites found in many plants. In Lonicera species, flavones contribute both physiological and pharmaceutical properties. However, flavone synthase (FNS), the key enzyme responsible for flavone biosynthesis, has not yet been characterized in Lonicera species. In this study, FNSII genes were identified from Lonicera japonica Thunb. and L. macranthoides Hand. Mazz. In the presence of NADPH, the recombinant cytochrome P450 proteins encoded by LjFNSII-1.1, LjFNSII-2.1, and LmFNSII-1.1 converted eriodictyol, naringenin, and liquiritigenin to the corresponding flavones directly. The different catalytic properties between LjFNSII-2.1 and LjFNSII-1.1 were caused by a single amino acid substitution at position 242 (glutamic acid to lysine). A methionine at position 206 and a leucine at position 381 contributed considerably to the high catalytic activity of LjFNSII-1.1. In addition, LjFNSII-1.1&2.1 and LmFNSII-1.1 also biosynthesize flavones that were further modified by O-glycosylation in transgenic tobacco. The expression levels of the FNSII genes were consistent with flavone accumulation patterns in flower buds. Our findings suggested that the weak catalytic activity of LmFNSII-1.1 and the relatively low expression of LmFNSII-1.1 in flowers might be responsible for the low levels of flavone accumulation in flower buds of L. macranthoides.
学科领域	Multidisciplinary Sciences
DOI	10.1038/srep19245
收录类别	SCI
语种	英语
WOS关键词	CYTOCHROME-P450 GENES ; INDUCIBLE FLAVONE ; IDENTIFICATION ; EXPRESSION ; BIOSYNTHESIS ; CLONING ; MAIZE ; GLYCOSYLATION ; 2-HYDROXYLASE ; LUTEOLIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000368108000002
出版者	NATURE PUBLISHING GROUP
文献子类	Article
出版地	LONDON
资助机构	National Science and Technology Basic Project of China [2014FY110100]
作者邮箱	shshli@ibcas.ac.cn ; wanglsh@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：45[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25385
专题	中科院植物分子生理学重点实验室
作者单位	1.[Wu, Jie 2.Liu, Yang 3.Du, Hui 4.Shu, Qing-Yan 5.Su, Shang 6.Wang, Li-Jin 7.Li, Shan-Shan 8.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Key Lab Plant Resources, Beijing 100093, Peoples R China 9.Wang, Xiao-Chen 10.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 11.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wu, Jie,Wang, Xiao-Chen,Liu, Yang,et al. Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation[J]. SCIENTIFIC REPORTS,2016,6.
APA	Wu, Jie.,Wang, Xiao-Chen.,Liu, Yang.,Du, Hui.,Shu, Qing-Yan.,...&Wang, Liang-Sheng.(2016).Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation.SCIENTIFIC REPORTS,6.
MLA	Wu, Jie,et al."Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation".SCIENTIFIC REPORTS 6(2016).

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