Bovine serum albumin in saliva mediates grazing response in Leymus chinensis rleevealed by RNA sequencing

doi:10.1186/1471-2164-15-1126

	Bovine serum albumin in saliva mediates grazing response in Leymus chinensis rleevealed by RNA sequencing
	Huang, Xin 1,2; Peng, Xianjun 1; Zhang, Lexin 3; Chen, Shuangyan ; Cheng, Liqin ; Liu, Gongshe
	2014
发表期刊	BMC GENOMICS
ISSN	1471-2164
卷号	15
摘要	Background: Sheepgrass (Leymus chinensis) is an important perennial forage grass across the Eurasian Steppe and is adaptable to various environmental conditions, but little is known about its molecular mechanism responding to grazing and BSA deposition. Because it has a large genome, RNA sequencing is expensive and impractical except for the next-generation sequencing (NGS) technology. Results: In this study, NGS technology was employed to characterize de novo the transcriptome of sheepgrass after defoliation and grazing treatments and to identify differentially expressed genes (DEGs) responding to grazing and BSA deposition. We assembled more than 47 M high-quality reads into 120,426 contigs from seven sequenced libraries. Based on the assembled transcriptome, we detected 2,002 DEGs responding to BSA deposition during grazing. Enrichment analysis of Gene ontology (GO), EuKaryotic Orthologous Groups (KOG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways revealed that the effects of grazing and BSA deposition involved more apoptosis and cell oxidative changes compared to defoliation. Analysis of DNA fragments, cell oxidative factors and the lengths of leaf scars after grazing provided physiological and morphological evidence that BSA deposition during grazing alters the oxidative and apoptotic status of cells. Conclusions: This research greatly enriches sheepgrass transcriptome resources and grazing-stress-related genes, helping us to better understand the molecular mechanism of grazing in sheepgrass. The grazing-stress-related genes and pathways will be a valuable resource for further gene-phenotype studies.
关键词	Leymus chinensis Grazing BSA deposition RNA-seq Apoptosis Cell oxidative status
学科领域	Biotechnology & Applied Microbiology ; Genetics & Heredity
DOI	10.1186/1471-2164-15-1126
收录类别	SCI
语种	英语
WOS关键词	SALT-INDUCED GENE ; LOLIUM-PERENNE L ; CELL-DEATH ; TRANSCRIPTOME ANALYSIS ; CARBOHYDRATE RESERVES ; GENOME ANNOTATION ; OXIDATIVE BURST ; DEFOLIATION ; EXPRESSION ; TOLERANCE
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000347726400001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Basic Research Program of China (973) [2014CB138704] ; National High Technology Research and Development Program of China (863) [2011AA100209] ; Project of Ningxia Agricultural Comprehensive Development Office [NTKJ -2013-04, NTKJ -2014-04]
作者邮箱	sychen@ibcas.ac.cn ; lqcheng@ibcas.ac.cn ; liugs@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27361
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.ShangHai Acad Agr Sci, Forest & Fruit Tree Res Inst, Shanghai, Peoples R China 4.Heze Entry Exit Inspect & Quarantine Bur, Jinan, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Huang, Xin,Peng, Xianjun,Zhang, Lexin,et al. Bovine serum albumin in saliva mediates grazing response in Leymus chinensis rleevealed by RNA sequencing[J]. BMC GENOMICS,2014,15.
APA	Huang, Xin,Peng, Xianjun,Zhang, Lexin,Chen, Shuangyan,Cheng, Liqin,&Liu, Gongshe.(2014).Bovine serum albumin in saliva mediates grazing response in Leymus chinensis rleevealed by RNA sequencing.BMC GENOMICS,15.
MLA	Huang, Xin,et al."Bovine serum albumin in saliva mediates grazing response in Leymus chinensis rleevealed by RNA sequencing".BMC GENOMICS 15(2014).

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