Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MSn

doi:10.1371/journal.pone.0062291

	Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MSn
	Chen, Sha 1,2,3; Xiang, Yue 3; Deng, Jiao 3; Liu, Yanling ; Li, Shaohua1,2
	2013
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:4
摘要	A validated HPLC-DAD-ESI-MSn method for the analysis of non-anthocyanin flavonoids was applied to nine different tissues of twelve lotus genotypes of Nelumbo nucifera and N. lutea, together with an optimized anthocyanin extraction and separation protocol for lotus petals. A total of five anthocyanins and twenty non-anthocyanin flavonoids was identified and quantified. Flavonoid contents and compositions varied with cultivar and tissue and were used as a basis to divide tissues into three groups characterized by kaempferol and quercetin derivatives. Influences on flower petal coloration were investigated by principal components analyses. High contents of kaempferol glycosides were detected in the petals of N. nucifera while high quercetin glycoside concentrations occurred in N. lutea. Based on these results, biosynthetic pathways leading to specific compounds in lotus tissues are deduced through metabolomic analysis of different genotypes and tissues and correlations among flavonoid compounds.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0062291
收录类别	SCI
语种	英语
WOS关键词	IONIZATION MASS-SPECTROMETRY ; NUCIFERA GAERTN. ; BIOSYNTHESIS ; CHROMATOGRAPHY ; LEAVES ; POLYPHENOLS ; FRUITS ; PETALS ; PARTS ; ACID
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000319077300038
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	Knowledge Innovation Project of the Chinese Academy of Sciences(Knowledge Innovation Program of the Chinese Academy of Sciences)
作者邮箱	liu, yan/HCI-5542-2022
作品OA属性	Green Submitted, gold, Green Published
引用统计	被引频次：47[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27803
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Bot, Lab Plant Resource, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Chen, Sha,Xiang, Yue,Deng, Jiao,et al. Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MSn[J]. PLOS ONE,2013,8(4).
APA	Chen, Sha,Xiang, Yue,Deng, Jiao,Liu, Yanling,&Li, Shaohua.(2013).Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MSn.PLOS ONE,8(4).
MLA	Chen, Sha,et al."Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MSn".PLOS ONE 8.4(2013).

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