A Medicago truncatula EF-Hand Family Gene, MtCaMP1, Is Involved in Drought and Salt Stress Tolerance

doi:10.1371/journal.pone.0058952

	A Medicago truncatula EF-Hand Family Gene, MtCaMP1, Is Involved in Drought and Salt Stress Tolerance
	Wang, Tian-Zuo ; Zhang, Jin-Li ; Tian, Qiu-Ying ; Zhao, Min-Gui ; Zhang, Wen-Hao
	2013
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:4
摘要	Background: Calcium-binding proteins that contain EF-hand motifs have been reported to play important roles in transduction of signals associated with biotic and abiotic stresses. To functionally characterize gens of EF-hand family in response to abiotic stress, an MtCaMP1 gene belonging to EF-hand family from legume model plant Medicago truncatula was isolated and its function in response to drought and salt stress was investigated by expressing MtCaMP1 in Arabidopsis. Methodology/Principal Findings: Transgenic Arabidopsis seedlings expressing MtCaMP1 exhibited higher survival rate than wild-type seedlings under drought and salt stress, suggesting that expression of MtCaMP1 confers tolerance of Arabidopsis to drought and salt stress. The transgenic plants accumulated greater amounts of Pro due to up-regulation of P5CS1 and down-regulation of ProDH than wild-type plants under drought stress. There was a less accumulation of Na+ in the transgenic plants than in WT plants due to reduced up-regulation of AtHKT1 and enhanced regulation of AtNHX1 in the transgenic plants compared to WT plants under salt stress. There was a reduced accumulation of H2O2 and malondialdehyde in the transgenic plants than in WT plants under both drought and salt stress. Conclusions/Significance: The expression of MtCaMP1 in Arabidopsis enhanced tolerance of the transgenic plants to drought and salt stress by effective osmo-regulation due to greater accumulation of Pro and by minimizing toxic Na+ accumulation, respectively. The enhanced accumulation of Pro and reduced accumulation of Na+ under drought and salt stress would protect plants from water default and Na+ toxicity, and alleviate the associated oxidative stress. These findings demonstrate that MtCaMP1 encodes a stress-responsive EF-hand protein that plays a regulatory role in response of plants to drought and salt stress.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0058952
收录类别	SCI
语种	英语
WOS关键词	ARABIDOPSIS-THALIANA ; PROTEIN-KINASES ; ABIOTIC STRESS ; SIGNAL-TRANSDUCTION ; CALCIUM ; PROLINE ; EXPRESSION ; CALMODULIN ; INDUCTION ; RADIATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000317898000006
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	State Key Lab of Vegetation and Environmental Change
作者邮箱	Wang, Tianzuo/C-7291-2013 ; Zhang, Wen-Hao/B-5805-2009
作品OA属性	Green Submitted, Green Published, Green Accepted, gold
引用统计	被引频次：62[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27829
专题	植被与环境变化国家重点实验室
作者单位	1.[Wang, Tian-Zuo 2.Zhang, Jin-Li 3.Tian, Qiu-Ying 4.Zhao, Min-Gui 5.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing, Peoples R China 6.Chinese Acad Sci, Beijing Inst Life Sci, Res Network Global Change Biol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Tian-Zuo,Zhang, Jin-Li,Tian, Qiu-Ying,et al. A Medicago truncatula EF-Hand Family Gene, MtCaMP1, Is Involved in Drought and Salt Stress Tolerance[J]. PLOS ONE,2013,8(4).
APA	Wang, Tian-Zuo,Zhang, Jin-Li,Tian, Qiu-Ying,Zhao, Min-Gui,&Zhang, Wen-Hao.(2013).A Medicago truncatula EF-Hand Family Gene, MtCaMP1, Is Involved in Drought and Salt Stress Tolerance.PLOS ONE,8(4).
MLA	Wang, Tian-Zuo,et al."A Medicago truncatula EF-Hand Family Gene, MtCaMP1, Is Involved in Drought and Salt Stress Tolerance".PLOS ONE 8.4(2013).

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