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Efficient CELI endonuclease production in Nicotiana benthamiana through transient expression and applications in detections of mutation and gene editing events 期刊论文
PLANT SCIENCE, 2020, 卷号: 296
Authors:  Yao, Xue-Feng;  Wu, Shengyang;  Guo, Lei;  Liu, Chun-Ming
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Production of CELI endonuclease  Optimization of CELI digestion  Transient expression  Detections of mismatch and gene editing  
Knockout of VvCCD8 gene in grapevine affects shoot branching 期刊论文
BMC PLANT BIOLOGY, 2020, 卷号: 20, 期号: 1
Authors:  Ren, Chong;  Guo, Yuchen;  Kong, Junhua;  Lecourieux, Fatma;  Dai, Zhanwu;  Li, Shaohua;  Liang, Zhenchang
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CRISPR  Cas9  CCD8  Mutant  Shoot branching  Strigolactone  
An efficient TILLING platform for cultivated tobacco 期刊论文
JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 2020, 卷号: 62, 期号: 2, 页码: 165-180
Authors:  Gao, Yu-Long;  Yao, Xue-Feng;  Li, Wen-Zheng;  Song, Zhong-Bang;  Wang, Bing-Wu;  Wu, Yu-Ping;  Shi, Jun-Li;  Liu, Guan-Shan;  Li, Yong-Ping;  Liu, Chun-Ming
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Recovery of the non-functional EGFP-assisted identification of mutants generated by CRISPR/Cas9 期刊论文
PLANT CELL REPORTS, 2019, 卷号: 38, 期号: 12, 页码: 1541-1549
Authors:  Ren, Chong;  Guo, Yuchen;  Gathunga, Elias Kirabi;  Duan, Wei;  Li, Shaohua;  Liang, Zhenchang
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Selection of mutants  CRISPR  Cas9  Non-functional EGFP  Reporter gene  
SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola 期刊论文
PLANT CELL AND ENVIRONMENT, 2019, 卷号: 42, 期号: 4, 页码: 1112-1124
Authors:  Zhao, Haixia;  Wang, Liangsheng;  Zhao, Fang-Jie;  Wu, Longhua;  Liu, Anna;  Xu, Wenzhong
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cadmium (Cd)  chloroplast  CRISPR/Cas9  heavy metal ATPase 1 (HMA1)  hyperaccumulator  Sedum plumbizincicola  transporter  
Improvement of multiplex semi-nested PCR system for screening of rare mutations by high-throughput sequencing 期刊论文
BIOTECHNIQUES, 2019, 卷号: 67, 期号: 6, 页码: 294-298
Authors:  Zhang, Yingchun;  Chi, Xu;  Feng, Laibao;  Wu, Xingwen;  Qi, Xiaoquan
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dilution  improvement  multiplexed target enrichment  mutants  next generation sequencing  
Efficiency Optimization of CRISPR/Cas9-Mediated Targeted Mutagenesis in Grape 期刊论文
FRONTIERS IN PLANT SCIENCE, 2019, 卷号: 10
Authors:  Ren, Fengrui;  Ren, Chong;  Zhang, Zhan;  Duan, Wei;  Lecourieux, David;  Li, Shaohua;  Liang, Zhenchang
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CRISPR/Cas9  optimization  GC content  grape  gene expression  gene editing efficiency  
CRISPR-Cas9-mediated genome editing in apple and grapevine 期刊论文
NATURE PROTOCOLS, 2018, 卷号: 13, 期号: 12, 页码: 2844-2863
Authors:  Osakabe, Yuriko;  Liang, Zhenchang;  Ren, Chong;  Nishitani, Chikako;  Osakabe, Keishi;  Wada, Masato;  Komori, Sadao;  Malnoy, Mickael;  Velasco, Riccardo;  Poli, Michele;  Jung, Min-Hee;  Koo, Ok-Jae;  Viola, Roberto;  Kanchiswamy, Chidananda Nagamangala
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基因编辑技术在莱茵衣藻中的应用进展 期刊论文
生命科学, 2018, 卷号: 30, 期号: 09, 页码: 987-993
Authors:  涂文凤;  王月;  杨文强
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莱茵衣藻  基因编辑  ZFN  TALEN  CRISPR/Cas9  CRISPR/Cpf1  
葡萄基因编辑的魔法“剪刀”——CRISPR/Cas9 期刊论文
生命世界, 2018, 期号: 04, 页码: 24-25
Authors:  段伟
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CRISPR/Cas9  基因编辑  基因编辑技术